Growth and exopolysaccharide (EPS) production by Oenococcus oeni I4 and structural characterization of their EPSs
نویسندگان
چکیده
منابع مشابه
Exopolysaccharide (EPS) Synthesis by Oenococcus oeni: From Genes to Phenotypes
Oenococcus oeni is the bacterial species which drives malolactic fermentation in wine. The analysis of 50 genomic sequences of O. oeni (14 already available and 36 newly sequenced ones) provided an inventory of the genes potentially involved in exopolysaccharide (EPS) biosynthesis. The loci identified are: two gene clusters named eps1 and eps2, three isolated glycoside-hydrolase genes named dsr...
متن کاملOenococcus oeni in Chilean Red Wines: Technological and Genomic Characterization
The presence and load of species of LAB at the end of the malolactic fermentation (MLF) were investigated in 16 wineries from the different Chilean valleys (Limarí, Casablanca, Maipo, Rapel, and Maule Valleys) during 2012 and 2013, using PCR-RFLP and qPCR. Oenococcus oeni was observed in 80% of the samples collected. Dominance of O. oeni was reflected in the bacterial load (O. oeni/total bacter...
متن کاملMalolactic enzyme from Oenococcus oeni
Malolactic enzymes (MLE) are known to directly convert L-malic acid into L-lactic acid with a catalytical requirement of nicotinamide adenine dinucleotide (NAD (+) ) and Mn ( 2+) ; however, the reaction mechanism is still unclear. To study a MLE, the structural gene from Oenococcus oeni strain DSM 20255 was heterologously expressed in Escherichia coli, yielding 22.9 kU l (-1) fermentation broth...
متن کاملcharacterization of extracellular polysaccharides (eps) produced by thermal bacillus and determination of environmental conditions affecting exopolysaccharide production
in this study, the neutral monosaccharide composition of the extracellular polysaccharide (eps), extracted from thermal bacillus, was determined by liquid chromatography-mass spectrometry and high performance liquid chromatography. our analysis indicated that the eps consisted of rhamnose, mannose, galactose, glucose, fructose, arabinose and xylose. in addition to the neutral sugars in the eps,...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2007
ISSN: 1364-5072,1365-2672
DOI: 10.1111/j.1365-2672.2006.03266.x